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DNA Sequencing Videohistory Collection

Extent:
5 videotapes (Reference copies). 22 digital .wmv files and .rm files (Reference copies).
Type:
Collection descriptions
Archival materials
Videotapes
Transcripts
Date:
1989-1990
Introduction:
The Smithsonian Videohistory Program, funded by the Alfred P. Sloan Foundation from 1986 until 1992, used video in historical research. Additional collections have been added since the grant project ended. Videohistory uses the video camera as a historical research tool to record moving visual information. Video works best in historical research when recording people at work in environments, explaining artifacts, demonstrating process, or in group discussion. The experimental program recorded projects that reflected the Institution's concern with the conduct of contemporary science and technology.

Smithsonian historians participated in the program to document visual aspects of their on-going historical research. Projects covered topics in the physical and biological sciences as well as in technological design and manufacture. To capture site, process, and interaction most effectively, projects were taped in offices, factories, quarries, laboratories, observatories, and museums. Resulting footage was duplicated, transcribed, and deposited in the Smithsonian Institution Archives for scholarship, education, and exhibition. The collection is open to qualified researchers.
Descriptive Entry:
Ramunas Kondratas, curator at the Smithsonian's National Museum of American History (NMAH), was interested in documenting the history, development, and applications of the DNA Sequencer. He also explored the commercialization of the instrument, including its testing and marketing, and addressed current and future uses of the ABI 370A model sequencer in medical research. Sessions were recorded at California Institute of Technology in Pasadena, California, on October 19, 1988, at Applied Biosystems, Inc., in Foster City, California, on October 21, 1988, and at the National Institutes of Health (NIH) in Washington, D.C., on March 27, 1990.

Interviewees included scientists and technicians at Cal Tech, ABI, and NIH. Jeannine Gocayne received a M.A. in molecular biology from the State University of New York-Buffalo in 1985 and was appointed a biologist and sequencing supervisor with the Receptor Biochemistry and Molecular Biology Section of the Laboratory of Molecular and Cellular Neurobiology, National Institute of Neurological Disorders and Stroke (NINDS), NIH in 1986.

Several others provided additional information about the sequencer for the three video sessions. These people included: Kurt Becker, DNA Sequencing Product Manager; Kip Connell, research scientist; Marilee Shaffer, products specialist for DNA sequencing, ABI; and Anthony R. Kerlavage and W. Richard McCombie of the Receptor Biochemistry and Molecular Biology Section of the Laboratory of Molecular and Cellular Neurobiology, NINDS, NIH.

Session one took place at the California Institute of Technology with Hood, Sanders, and Kaiser. Interviews focused on the history, design, and development of the sequencer prototype and its operation.

Session Two took place at Applied Biosystems, Inc., with Hunkapiller, Becker, Connell, and Shaffer and dealt with the commercial design, fabrication, and marketing of the sequencer and other related instrumentation. Tours of the assembly and manufacturing areas were included in the session, as well as a demonstration of how the DNA sequencing data is represented graphically on a computer.

Session Three took place at the Receptor Biochemistry and Molecular Biology Section of the Laboratory of Molecular and Cellular Neurobiology, NINDS, NIH, where Venter explained and demonstrated the automated DNA sequencing processes during a tour of the lab. Kerlavage and McCombie assisted during the tour. Finally, Gocayne described the application of new DNA sequencing technology to work in the lab.

This collection consists of three interview sessions, totaling approximately 8:40 hours of recordings and 176 pages of transcript.
Historical Note:
DNA is composed of the four individual nucleotides: adenine (A), thymine (T), cytosine (C), and guanine (G). To decipher a particular piece of DNA, it is necessary to determine the exact sequence of these nucleotides. The sequence of the nucleotides determines the genetic information encoded in a DNA strand. A partial nucleotide sequence for a human gene might look like: GGCACTGACTCTCTC. In 1977, biochemist Fred Sanger developed the enzymatic chain termination procedure that allowed for sequencing of individual strands of DNA. This made mapping and sequencing of genetic material possible.

In 1986, Leroy E. Hood's Laboratory at the California Institute of Technology (Cal Tech) announced its development of a semiautomated machine for sequencing DNA. The machine automated the enzymatic chain termination procedure for DNA sequence analysis developed by Sanger and became a key instrument in mapping and sequencing genetic material. That same year, Applied Biosystems, Inc. (ABI) produced the first commercial instruments for clinical use. Constant improvements in the technology resulted in faster sequencing capacity, which was significant for advanced scientific research in projects such as mapping the human genome.

Leroy E. Hood received his M.D. from The Johns Hopkins School of Medicine in 1964, and a Ph.D. in immunology from Cal Tech in 1968. From 1968 until 1970 he held a postdoctoral fellowship at the National Institutes of Health. In 1970 he was appointed professor of biology at Cal Tech and eventually became chairman of the Division of Biology and the director of its cancer center.

Michael Hunkapiller received a Ph.D. in chemistry from Cal Tech in 1974. He joined ABI as its vice president for research and development in 1983.

Robert J. Kaiser received his Ph.D in chemistry from Cal Tech in 1983, and subsequently joined the Cal Tech staff as a research fellow in biology. Jane Z. Sanders joined the Cal Tech staff in 1984 as an associate biologist and was appointed senior biologist a year later. She took graduate courses in biochemistry in 1971-1972 at the Stanford University Medical School.

Lloyd M. Smith received a Ph.D. in biophysics from Stanford University in 1981, and was a senior research fellow in biology at Cal Tech from 1982 until 1987, when he was appointed assistant professor in the Analytical Division of the Department of Chemistry at the University of Wisconsin-Madison.

J. Craig Venter received his Ph.D. in physiology and pharmacology from the University of California, San Diego in 1975. In 1983 he was appointed adjunct professor of biochemical pharmacology at the State University of New York-Buffalo and joined NIH in 1984 as chief of the Receptor Biochemistry and Molecular Biology Section, NINDS. In 1987 he also became co-director of the Laboratory of Molecular and Cellular Neurobiology, NINDS, NIH and was appointed director of the NINDS DNA facility at NIH.
Rights:
Restricted. The permission of Ramunas Kondratas must be obtained before the public broadcast or public viewing of the tapes. Contact SIHistory@si.edu for more details.
Topic:
Sequence alignment (Bioinformatics)  Search this
Science -- History  Search this
Molecular biology  Search this
Laboratories  Search this
Nucleic acids -- Analysis  Search this
Nucleotides -- Analysis  Search this
Nucleotide sequence  Search this
Interviews  Search this
Oral history  Search this
Technology -- History  Search this
DNA -- Analysis  Search this
Genre/Form:
Videotapes
Transcripts
Citation:
Smithsonian Institution Archives, Record Unit 9549, DNA Sequencing Videohistory Collection
Identifier:
Record Unit 9549
See more items in:
DNA Sequencing Videohistory Collection
Archival Repository:
Smithsonian Institution Archives
EDAN-URL:
ead_collection:sova-sia-faru9549

3700 DNA Analyzer

Maker:
Applied Biosystems  Search this
Physical Description:
steel (overall material)
plastic (overall material)
Measurements:
overall: 53 in x 30 in x 29 1/2 in; 134.62 cm x 76.2 cm x 74.93 cm
Object Name:
DNA analyzer
Date made:
1999
Credit Line:
Applied Biosystems, Genetic Analysis Division (through Deborah A. Smeltzer)
ID Number:
2004.0226.1
Accession number:
2004.0226
Catalog number:
2004.0226.01
See more items in:
Medicine and Science: Biological Sciences
Science & Mathematics
Biotechnology and Genetics
Data Source:
National Museum of American History
GUID:
http://n2t.net/ark:/65665/ng49ca746ab-c7e7-704b-e053-15f76fa0b4fa
EDAN-URL:
edanmdm:nmah_1297334
Online Media:

Migration of endpoints of two genes relative to boundaries between regions of the plastid genome in the grass family (Poaceae)

Author:
Davis, Jerrold I.  Search this
Soreng, Robert J.  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2010
Topic:
Botany  Search this
Plants  Search this
Natural History  Search this
See others in:
Botany
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_82024

Redesign of PCR primers for mitochondrial cytochrome c oxidase subunit I for marine invertebrates and application in all-taxa biotic surveys

Author:
Hawk, H.  Search this
Meyer, Christopher P.  Search this
Geller, J.  Search this
Parker, M.  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2013
Topic:
Invertebrates  Search this
Animals  Search this
Zoology  Search this
Natural History  Search this
See others in:
Invertebrate Zoology
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_116487

Evolution of a Complex Disease Resistance Gene Cluster in Diploid Phaseolus and Tetraploid Glycine

Author:
Pfeil, Bernard E.  Search this
Chen, Nicolas W. G.  Search this
Ameline-Torregrosa, Carine  Search this
Young, Nevin D.  Search this
Geffroy, Valérie  Search this
Doyle, Jeff J.  Search this
Denny, Roxanne  Search this
Roe, Bruce A.  Search this
Podicheti, Ram  Search this
Ratnaparkhe, Milind B.  Search this
Ashfield, Tom  Search this
Cannon, Steven  Search this
Egan, Ashley N.  Search this
Maroof, M. A. S.  Search this
Innes, Roger W.  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2012
Topic:
Botany  Search this
Plants  Search this
Natural History  Search this
See others in:
Botany
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_118254

A comparative study of nemertean complete mitochondrial genomes, including two new ones for Nectonemertes cf. mirabilis and Zygeupolia rubens, may elucidate the fundamental pattern for the phylum Nemertea

Author:
Ren, Wei-Cheng  Search this
Norenburg, Jon L.  Search this
Sun, Shi-Chun  Search this
Sundberg, Per  Search this
Chen, Hai-Xia  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2012
Topic:
Invertebrates  Search this
Animals  Search this
Zoology  Search this
Natural History  Search this
See others in:
Invertebrate Zoology
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_111340

Multiple Independent Origins of Mitochondrial Control Region Duplications in the Order Psittaciformes

Author:
Müeller, Heinrich  Search this
Fleischer, Robert C.  Search this
Tavares, Erika S.  Search this
Eberhard, Jessica R.  Search this
Miyaki, Cristina Y.  Search this
Hernandez, Alexis  Search this
Wright, Timothy F.  Search this
Graves, Gary R.  Search this
Gonzales, Lauren A.  Search this
Sanchez, Juan J.  Search this
Schirtzinger, Erin E.  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2012
Topic:
Vertebrates  Search this
Animals  Search this
Zoology  Search this
Natural History  Search this
Zoology  Search this
Animals  Search this
Veterinary medicine  Search this
Animal health  Search this
See others in:
Vertebrate Zoology
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_111395

The mitochondrial genome of the Cinnamon Bittern, Ixobrychus cinnamomeus (Pelecaniformes: Ardeidae): sequence, structure and phylogenetic analysis

Author:
Zhang, Liqin  Search this
Kan, Xianzhao  Search this
Gowda, Vinita  Search this
Wang, Ming  Search this
Wang, Li  Search this
Li, Xifeng  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2012
Topic:
Botany  Search this
Plants  Search this
Natural History  Search this
See others in:
Botany
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_111937

Next-generation sequencing reveals phylogeographic structure and a species tree for recent bird divergences

Author:
McCormack, John E.  Search this
Hird, Sarah M.  Search this
Graves, Gary R.  Search this
Brumfield, Robb T.  Search this
Derryberry, Elizabeth P.  Search this
Maley, James M.  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2012
Topic:
Vertebrates  Search this
Animals  Search this
Zoology  Search this
Natural History  Search this
See others in:
Vertebrate Zoology
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_109760

Genetic and morphological evidence that the Eastern Pacific damselfish Abudefduf declivfrons is distinct from A. concolor (Pomacentridae)

Author:
Lessios, Harilaos A.  Search this
Wellington, Gerard M.  Search this
Bermingham, Eldredge  Search this
Allen, Gerald R.  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
1995
Topic:
Tropics  Search this
Biology  Search this
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_103836

Can Deliberately Incomplete Gene Sample Augmentation Improve a Phylogeny Estimate for the Advanced Moths and Butterflies (Hexapoda: Lepidoptera)?

Author:
Regier, Jerome C.  Search this
Zwick, Andreas  Search this
Davis, Donald R.  Search this
Mitter, Charles  Search this
Weller, Susan  Search this
Parr, Cynthia Sims  Search this
Cho, Soowon  Search this
Yao, Jianxiu  Search this
Du, Zaile  Search this
Cummings, Michael P.  Search this
Brown, John W.  Search this
Zhao, Hong  Search this
Kawahara, Akito Y.  Search this
Baixeras, Joaquin  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2011
Topic:
Entomology  Search this
Insects  Search this
Natural History  Search this
See others in:
Entomology
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_103963

Complete nucleotide sequences of the domestic cat (Felis catus) mitochondrial genome and a transposed mtDNA tandem repeat (Numt) in the nuclear genome

Author:
Lopez, Jose V.  Search this
O'Brien, Sean T.  Search this
Cevario, Stanley  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
1996
Topic:
Tropics  Search this
Biology  Search this
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_103583

A molecular phylogeny of the Gelidiales (Rhodophyta) based on analysis of plastid rbcL nucleotide sequences

Author:
Freshwater, D. W.  Search this
Fredericq, Suzanne  Search this
Hommersand, M. J.  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
1995
Topic:
Natural History  Search this
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_98582

A non-native bryozoan creates novel substrate on the mudflats in San Francisco Bay

Author:
Gentry, Jackson  Search this
Obernolte, Rena  Search this
Geller, Jonathan  Search this
Mackie, Joshua A.  Search this
Harris, Leslie  Search this
Zabin, Chela J.  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2010
Topic:
Animal health  Search this
Environmental sciences  Search this
Coastal ecology  Search this
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_92610

Statistical Parsimony Networks and Species Assemblages in Cephalotrichid Nemerteans (Nemertea)

Author:
Norenburg, Jon L.  Search this
Sun, Shichun  Search this
Strand, Malin  Search this
Kajihara, Hiroshi  Search this
Maslakova, Svetlana A.  Search this
Sundberg, Per  Search this
Chernyshev, Alexey V.  Search this
Chen, Haixia  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2010
Topic:
Natural History  Search this
Invertebrates  Search this
Animals  Search this
Zoology  Search this
See others in:
Invertebrate Zoology
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_92611

It's not too Late for the Harpy Eagle (Harpia harpyja): High Levels Of Genetic Diversity and Differentiation Can Fuel Conservation Programs

Author:
Mindell, David P.  Search this
Lindsay, Alec R.  Search this
Lerner, Heather R. L.  Search this
Johnson, Jeff A.  Search this
Kiff, Lloyd F.  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2009
Topic:
Zoology  Search this
Animals  Search this
Veterinary medicine  Search this
Animal health  Search this
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_81801

The evolution of food preferences in Coccinellidae

Author:
Whiting, Michael F.  Search this
Forrester, Juanita A.  Search this
Shapiro, Lori R.  Search this
Vandenberg, Natalia J.  Search this
Slipinski, S. Adam  Search this
Mchugh, Joseph V.  Search this
Giorgi, José Adriano  Search this
Miller, Kelly B.  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2009
Topic:
Entomology  Search this
Insects  Search this
Natural History  Search this
See others in:
Entomology
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_80180

Phylogenetic and biogeographic analysis of the Sparidae (Perciformes : Percoidei) from cytochrome b sequences

Author:
Orrell, Thomas M.  Search this
Musick, John A.  Search this
Graves, John E.  Search this
Carpenter, Kent E.  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2002
Topic:
Natural History  Search this
Vertebrates  Search this
Animals  Search this
Zoology  Search this
See others in:
Vertebrate Zoology
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_74819

Phytochemical and genetic analyses of ancient cannabis from Central Asia

Author:
Bera, Subir  Search this
Zhao, Liang-Cheng  Search this
Jiang, Hong-En  Search this
Sutton, Alan  Search this
Liu, Chang-Jiang  Search this
Russo, Ethan B.  Search this
Lu, En-Guo  Search this
del Bianco, Francesca  Search this
Wang, Yu-Fei  Search this
Ferguson, David K.  Search this
Hueber, Francis M.  Search this
Li, Cheng-Sen  Search this
Carboni, Andrea  Search this
Zhao, You-Xing  Search this
Li, Xiao  Search this
Potter, David J.  Search this
Mandolino, Giuseppe  Search this
Zhang, Yong-Bing  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2008
Topic:
Natural History  Search this
Paleobiology  Search this
See others in:
Paleobiology
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_76638

DNA barcoding in land plants: developing standards to quantify and maximize success

Author:
Erickson, David L.  Search this
Spouge, John  Search this
Resch, Alissa  Search this
Weigt, Lee A.  Search this
Kress, W. John  Search this
Object Type:
Smithsonian staff publication
Electronic document
Year:
2008
Topic:
Natural History  Search this
Botany  Search this
Plants  Search this
See others in:
Botany
Data source:
Smithsonian Libraries
EDAN-URL:
edanmdm:SILSRO_76920

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